bacterial organism staphylococcus epidermidis (ATCC)
Structured Review

Bacterial Organism Staphylococcus Epidermidis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1342 article reviews
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1) Product Images from "Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria"
Article Title: Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria
Journal: Pathogens
doi: 10.3390/pathogens11020209
Figure Legend Snippet: Pathogenic P. aeruginosa (red columns) induces significant ( p < 0.05) cell death in A549 wild-type (WT) and engineered (ERK-Fra1) cells compared to non-pathogenic S. epidermidis (black columns) as measured by the alamarBlue assay. The positive control for cell death was 70% ethanol, resulting in less than 10% survival, and the positive controls for viability were LB and BHI medium, resulting in 100% survival. Each column represents the mean of three experiments ± standard deviation. Comparisons were made using a Student’s t-test and asterisks indicate significant differences (** p < 0.01, **** p < 0.0001).
Techniques Used: Alamar Blue Assay, Positive Control, Standard Deviation
Figure Legend Snippet: A549 ERK-Fra1 cells in response to ( A ) S. epidermidis , ( B ) A. baylyi , ( C ) S. agalactiae , ( D ) K. pneumoniae , and ( E ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. Cells have a strong Fra1 signal observed in yellow (mVenus, indicated by arrows) after S. epidermidis inoculation (panel A ). Cells have reduced Fra1 signaling (mVenus) by 4 h after pathogen inoculation (panels C – E ). Each organism was tested in at least triplicate. Image analysis of the Fra1 (mVenus) signal for all images can be found in . Constitutive ERK signaling is displayed in red (mCherry). Scale bar is 100 µm.
Techniques Used: Infection
Figure Legend Snippet: A549 wild-type (WT) cells in response to ( A ) EGF, ( B ) S. epidermidis , ( C ) A. baylyi , ( D ) S. agalactiae , ( E ) K. pneumoniae , and ( F ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. No significant changes in A549 WT cell phenotype were observed with treatment of EGF or S. epidermidis up to 12 h post-infection. Conversely, A549 WT show loss of adherence and tight junctions and the onset of apoptosis in response to the pathogens A. baylyi , S. agalactiae , K. pneumoniae, and P. aeruginosa . Each treatment or organism was tested in at least triplicate. Scale bar is 100 µm.
Techniques Used: Infection
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