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bacterial organism staphylococcus epidermidis  (ATCC)


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    Structured Review

    ATCC bacterial organism staphylococcus epidermidis
    Pathogenic P. aeruginosa (red columns) induces significant ( p < 0.05) cell death in A549 wild-type (WT) and engineered (ERK-Fra1) cells compared to non-pathogenic S. <t>epidermidis</t> (black columns) as measured by the alamarBlue assay. The positive control for cell death was 70% ethanol, resulting in less than 10% survival, and the positive controls for viability were LB and BHI medium, resulting in 100% survival. Each column represents the mean of three experiments ± standard deviation. Comparisons were made using a Student’s t-test and asterisks indicate significant differences (** p < 0.01, **** p < 0.0001).
    Bacterial Organism Staphylococcus Epidermidis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial organism staphylococcus epidermidis/product/ATCC
    Average 99 stars, based on 1342 article reviews
    bacterial organism staphylococcus epidermidis - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria"

    Article Title: Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria

    Journal: Pathogens

    doi: 10.3390/pathogens11020209

    Pathogenic P. aeruginosa (red columns) induces significant ( p < 0.05) cell death in A549 wild-type (WT) and engineered (ERK-Fra1) cells compared to non-pathogenic S. epidermidis (black columns) as measured by the alamarBlue assay. The positive control for cell death was 70% ethanol, resulting in less than 10% survival, and the positive controls for viability were LB and BHI medium, resulting in 100% survival. Each column represents the mean of three experiments ± standard deviation. Comparisons were made using a Student’s t-test and asterisks indicate significant differences (** p < 0.01, **** p < 0.0001).
    Figure Legend Snippet: Pathogenic P. aeruginosa (red columns) induces significant ( p < 0.05) cell death in A549 wild-type (WT) and engineered (ERK-Fra1) cells compared to non-pathogenic S. epidermidis (black columns) as measured by the alamarBlue assay. The positive control for cell death was 70% ethanol, resulting in less than 10% survival, and the positive controls for viability were LB and BHI medium, resulting in 100% survival. Each column represents the mean of three experiments ± standard deviation. Comparisons were made using a Student’s t-test and asterisks indicate significant differences (** p < 0.01, **** p < 0.0001).

    Techniques Used: Alamar Blue Assay, Positive Control, Standard Deviation

    A549 ERK-Fra1 cells in response to ( A ) S. epidermidis , ( B ) A. baylyi , ( C ) S. agalactiae , ( D ) K. pneumoniae , and ( E ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. Cells have a strong Fra1 signal observed in yellow (mVenus, indicated by arrows) after S. epidermidis inoculation (panel A ). Cells have reduced Fra1 signaling (mVenus) by 4 h after pathogen inoculation (panels C – E ). Each organism was tested in at least triplicate. Image analysis of the Fra1 (mVenus) signal for all images can be found in . Constitutive ERK signaling is displayed in red (mCherry). Scale bar is 100 µm.
    Figure Legend Snippet: A549 ERK-Fra1 cells in response to ( A ) S. epidermidis , ( B ) A. baylyi , ( C ) S. agalactiae , ( D ) K. pneumoniae , and ( E ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. Cells have a strong Fra1 signal observed in yellow (mVenus, indicated by arrows) after S. epidermidis inoculation (panel A ). Cells have reduced Fra1 signaling (mVenus) by 4 h after pathogen inoculation (panels C – E ). Each organism was tested in at least triplicate. Image analysis of the Fra1 (mVenus) signal for all images can be found in . Constitutive ERK signaling is displayed in red (mCherry). Scale bar is 100 µm.

    Techniques Used: Infection

    A549 wild-type (WT) cells in response to ( A ) EGF, ( B ) S. epidermidis , ( C ) A. baylyi , ( D ) S. agalactiae , ( E ) K. pneumoniae , and ( F ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. No significant changes in A549 WT cell phenotype were observed with treatment of EGF or S. epidermidis up to 12 h post-infection. Conversely, A549 WT show loss of adherence and tight junctions and the onset of apoptosis in response to the pathogens A. baylyi , S. agalactiae , K. pneumoniae, and P. aeruginosa . Each treatment or organism was tested in at least triplicate. Scale bar is 100 µm.
    Figure Legend Snippet: A549 wild-type (WT) cells in response to ( A ) EGF, ( B ) S. epidermidis , ( C ) A. baylyi , ( D ) S. agalactiae , ( E ) K. pneumoniae , and ( F ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. No significant changes in A549 WT cell phenotype were observed with treatment of EGF or S. epidermidis up to 12 h post-infection. Conversely, A549 WT show loss of adherence and tight junctions and the onset of apoptosis in response to the pathogens A. baylyi , S. agalactiae , K. pneumoniae, and P. aeruginosa . Each treatment or organism was tested in at least triplicate. Scale bar is 100 µm.

    Techniques Used: Infection



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    ATCC bacterial organism staphylococcus epidermidis
    Pathogenic P. aeruginosa (red columns) induces significant ( p < 0.05) cell death in A549 wild-type (WT) and engineered (ERK-Fra1) cells compared to non-pathogenic S. <t>epidermidis</t> (black columns) as measured by the alamarBlue assay. The positive control for cell death was 70% ethanol, resulting in less than 10% survival, and the positive controls for viability were LB and BHI medium, resulting in 100% survival. Each column represents the mean of three experiments ± standard deviation. Comparisons were made using a Student’s t-test and asterisks indicate significant differences (** p < 0.01, **** p < 0.0001).
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    Image Search Results


    Pathogenic P. aeruginosa (red columns) induces significant ( p < 0.05) cell death in A549 wild-type (WT) and engineered (ERK-Fra1) cells compared to non-pathogenic S. epidermidis (black columns) as measured by the alamarBlue assay. The positive control for cell death was 70% ethanol, resulting in less than 10% survival, and the positive controls for viability were LB and BHI medium, resulting in 100% survival. Each column represents the mean of three experiments ± standard deviation. Comparisons were made using a Student’s t-test and asterisks indicate significant differences (** p < 0.01, **** p < 0.0001).

    Journal: Pathogens

    Article Title: Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria

    doi: 10.3390/pathogens11020209

    Figure Lengend Snippet: Pathogenic P. aeruginosa (red columns) induces significant ( p < 0.05) cell death in A549 wild-type (WT) and engineered (ERK-Fra1) cells compared to non-pathogenic S. epidermidis (black columns) as measured by the alamarBlue assay. The positive control for cell death was 70% ethanol, resulting in less than 10% survival, and the positive controls for viability were LB and BHI medium, resulting in 100% survival. Each column represents the mean of three experiments ± standard deviation. Comparisons were made using a Student’s t-test and asterisks indicate significant differences (** p < 0.01, **** p < 0.0001).

    Article Snippet: The bacterial organism Staphylococcus epidermidis (ATCC 14990) and Klebsiella pneumoniae (NIST0151) were cultured in nutrient broth (BD Biosciences) at 37 °C with shaking at 200 rpm.

    Techniques: Alamar Blue Assay, Positive Control, Standard Deviation

    A549 ERK-Fra1 cells in response to ( A ) S. epidermidis , ( B ) A. baylyi , ( C ) S. agalactiae , ( D ) K. pneumoniae , and ( E ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. Cells have a strong Fra1 signal observed in yellow (mVenus, indicated by arrows) after S. epidermidis inoculation (panel A ). Cells have reduced Fra1 signaling (mVenus) by 4 h after pathogen inoculation (panels C – E ). Each organism was tested in at least triplicate. Image analysis of the Fra1 (mVenus) signal for all images can be found in . Constitutive ERK signaling is displayed in red (mCherry). Scale bar is 100 µm.

    Journal: Pathogens

    Article Title: Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria

    doi: 10.3390/pathogens11020209

    Figure Lengend Snippet: A549 ERK-Fra1 cells in response to ( A ) S. epidermidis , ( B ) A. baylyi , ( C ) S. agalactiae , ( D ) K. pneumoniae , and ( E ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. Cells have a strong Fra1 signal observed in yellow (mVenus, indicated by arrows) after S. epidermidis inoculation (panel A ). Cells have reduced Fra1 signaling (mVenus) by 4 h after pathogen inoculation (panels C – E ). Each organism was tested in at least triplicate. Image analysis of the Fra1 (mVenus) signal for all images can be found in . Constitutive ERK signaling is displayed in red (mCherry). Scale bar is 100 µm.

    Article Snippet: The bacterial organism Staphylococcus epidermidis (ATCC 14990) and Klebsiella pneumoniae (NIST0151) were cultured in nutrient broth (BD Biosciences) at 37 °C with shaking at 200 rpm.

    Techniques: Infection

    A549 wild-type (WT) cells in response to ( A ) EGF, ( B ) S. epidermidis , ( C ) A. baylyi , ( D ) S. agalactiae , ( E ) K. pneumoniae , and ( F ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. No significant changes in A549 WT cell phenotype were observed with treatment of EGF or S. epidermidis up to 12 h post-infection. Conversely, A549 WT show loss of adherence and tight junctions and the onset of apoptosis in response to the pathogens A. baylyi , S. agalactiae , K. pneumoniae, and P. aeruginosa . Each treatment or organism was tested in at least triplicate. Scale bar is 100 µm.

    Journal: Pathogens

    Article Title: Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria

    doi: 10.3390/pathogens11020209

    Figure Lengend Snippet: A549 wild-type (WT) cells in response to ( A ) EGF, ( B ) S. epidermidis , ( C ) A. baylyi , ( D ) S. agalactiae , ( E ) K. pneumoniae , and ( F ) P. aeruginosa at 0, 4, 8, and 12 h post-infection. No significant changes in A549 WT cell phenotype were observed with treatment of EGF or S. epidermidis up to 12 h post-infection. Conversely, A549 WT show loss of adherence and tight junctions and the onset of apoptosis in response to the pathogens A. baylyi , S. agalactiae , K. pneumoniae, and P. aeruginosa . Each treatment or organism was tested in at least triplicate. Scale bar is 100 µm.

    Article Snippet: The bacterial organism Staphylococcus epidermidis (ATCC 14990) and Klebsiella pneumoniae (NIST0151) were cultured in nutrient broth (BD Biosciences) at 37 °C with shaking at 200 rpm.

    Techniques: Infection

    Efficacy of Kan-AuNPs against bacterial strains [μg/ml].

    Journal: Frontiers in Microbiology

    Article Title: Novel Synthesis of Kanamycin Conjugated Gold Nanoparticles with Potent Antibacterial Activity

    doi: 10.3389/fmicb.2016.00607

    Figure Lengend Snippet: Efficacy of Kan-AuNPs against bacterial strains [μg/ml].

    Article Snippet: The bacterial organisms Staphylococcus epidermidis (ATCC #12228), Streptococcus bovis (ATCC # 9809), Enterobacter aerogenes (ATCC # 13048), Pseudomonas aeruginosa PA01, P. aeruginosa UNC-D , Yersinia pestis CO92 Lux P tolC pCD1 (-) , and Y. pestis CO92::Km Lux P tolC pCD1 (-) ( Y. pestis CO92 Lux P tolC pCD1 (-) with unresolved Kan resistance cassette) were cultured by standard procedures.

    Techniques:

    Efficacy of citrate-AuNPs compared to Kan-AuNPs [μg/ml a ].

    Journal: Frontiers in Microbiology

    Article Title: Novel Synthesis of Kanamycin Conjugated Gold Nanoparticles with Potent Antibacterial Activity

    doi: 10.3389/fmicb.2016.00607

    Figure Lengend Snippet: Efficacy of citrate-AuNPs compared to Kan-AuNPs [μg/ml a ].

    Article Snippet: The bacterial organisms Staphylococcus epidermidis (ATCC #12228), Streptococcus bovis (ATCC # 9809), Enterobacter aerogenes (ATCC # 13048), Pseudomonas aeruginosa PA01, P. aeruginosa UNC-D , Yersinia pestis CO92 Lux P tolC pCD1 (-) , and Y. pestis CO92::Km Lux P tolC pCD1 (-) ( Y. pestis CO92 Lux P tolC pCD1 (-) with unresolved Kan resistance cassette) were cultured by standard procedures.

    Techniques:

    Transmission electron microscope (TEM) images which visualize the morphological changes in bacteria upon treating with Kan-AuNPs at different intervals of time. (A) Represents sequential images (from left to right) of Gram-positive, S. epidermidis bacteria treated with Kan-AuNPs (18.00 μg mL -1 ) after 0, 6, and 12 h of incubation. (B) Represents sequential images (from left to right) of Gram-negative, E. aerogenes bacteria treated with Kan-AuNPs (16.00 μg mL -1 ) after 0, 6, and 12 h of incubation. After 6 h of exposure, Kan-AuNPs were found to adhere and penetrate the bacterial cell wall which resulted in disruption of cellular environment leading to lysis of cell due to leakage of cellular components as observed after 12 h of exposure. The results were similar for both Gram-positive and Gram-negative bacteria.

    Journal: Frontiers in Microbiology

    Article Title: Novel Synthesis of Kanamycin Conjugated Gold Nanoparticles with Potent Antibacterial Activity

    doi: 10.3389/fmicb.2016.00607

    Figure Lengend Snippet: Transmission electron microscope (TEM) images which visualize the morphological changes in bacteria upon treating with Kan-AuNPs at different intervals of time. (A) Represents sequential images (from left to right) of Gram-positive, S. epidermidis bacteria treated with Kan-AuNPs (18.00 μg mL -1 ) after 0, 6, and 12 h of incubation. (B) Represents sequential images (from left to right) of Gram-negative, E. aerogenes bacteria treated with Kan-AuNPs (16.00 μg mL -1 ) after 0, 6, and 12 h of incubation. After 6 h of exposure, Kan-AuNPs were found to adhere and penetrate the bacterial cell wall which resulted in disruption of cellular environment leading to lysis of cell due to leakage of cellular components as observed after 12 h of exposure. The results were similar for both Gram-positive and Gram-negative bacteria.

    Article Snippet: The bacterial organisms Staphylococcus epidermidis (ATCC #12228), Streptococcus bovis (ATCC # 9809), Enterobacter aerogenes (ATCC # 13048), Pseudomonas aeruginosa PA01, P. aeruginosa UNC-D , Yersinia pestis CO92 Lux P tolC pCD1 (-) , and Y. pestis CO92::Km Lux P tolC pCD1 (-) ( Y. pestis CO92 Lux P tolC pCD1 (-) with unresolved Kan resistance cassette) were cultured by standard procedures.

    Techniques: Transmission Assay, Microscopy, Bacteria, Incubation, Disruption, Lysis

    Fluorescence images of Kan-AuNP induced cell membrane permeability using propidium iodide (PI) dye. Upper panel represents Gram-positive bacteria, S. epidermidis , and lower panel represents Gram-negative bacteria, E. aerogenes . (A) For each image, the left half represents differential interference contrast mode, while the right half represents the corresponding fluorescence image. Untreated samples of respective bacteria with Kan-AuNPs were taken as control. (B) Represents a plot showing percentage permeability of S. epidermidis and E. aerogenes bacterial cells in presence and absence (Control) of Kan-AuNPs. ∗∗∗∗∗ p ≤ 0.00001 (Student’s t -test, two tailed assuming unequal variances).

    Journal: Frontiers in Microbiology

    Article Title: Novel Synthesis of Kanamycin Conjugated Gold Nanoparticles with Potent Antibacterial Activity

    doi: 10.3389/fmicb.2016.00607

    Figure Lengend Snippet: Fluorescence images of Kan-AuNP induced cell membrane permeability using propidium iodide (PI) dye. Upper panel represents Gram-positive bacteria, S. epidermidis , and lower panel represents Gram-negative bacteria, E. aerogenes . (A) For each image, the left half represents differential interference contrast mode, while the right half represents the corresponding fluorescence image. Untreated samples of respective bacteria with Kan-AuNPs were taken as control. (B) Represents a plot showing percentage permeability of S. epidermidis and E. aerogenes bacterial cells in presence and absence (Control) of Kan-AuNPs. ∗∗∗∗∗ p ≤ 0.00001 (Student’s t -test, two tailed assuming unequal variances).

    Article Snippet: The bacterial organisms Staphylococcus epidermidis (ATCC #12228), Streptococcus bovis (ATCC # 9809), Enterobacter aerogenes (ATCC # 13048), Pseudomonas aeruginosa PA01, P. aeruginosa UNC-D , Yersinia pestis CO92 Lux P tolC pCD1 (-) , and Y. pestis CO92::Km Lux P tolC pCD1 (-) ( Y. pestis CO92 Lux P tolC pCD1 (-) with unresolved Kan resistance cassette) were cultured by standard procedures.

    Techniques: Fluorescence, Membrane, Permeability, Bacteria, Control, Two Tailed Test